Colletotrichum coccodes (Wallr.) S. Hughes is a polyphagous fungus that infects both roots and aerial parts of plants from multiple botanical families, causing substantial economic losses in temperate, tropical, and subtropical regions. It most commonly occurs on peppers, pumpkins, and tomatoes, where it causes root rot, defoliation, blossom rot, and fruit rot depending on the infected organ. Effective risk assessment requires knowledge of the pathogen’s biology and etiology, as well as the selection of appropriate control strategies. Laboratory experiments were conducted at the Department of Plant Protection at the University of Life Sciences in Lublin to determine the growth rates of selected C. coccodes isolates on various culture media (PDA, MEA, CMA, CD), and to examine the effects of temperature (0°C, 5°C, 10°C, 15°C, 20°C, 25°C, 30°C) on colony development and sporulation. The results identified 25°C as the optimal temperature for C. coccodes growth while 0°C strongly inhibited development but was not lethal. Colony growth was fastest on potato dextrose agar (PDA), although sporulation was limited, while growth was slowest on Czapek-Dox (CD), where sporulation was abundant.