ISSR MARKERS IN THE GENETIC DIVERSITY OF
CHRYSANTHEMUM PLANTS DERIVED VIA SOMATIC
EMBRYOGENESIS
1
UTP University of Science and Technology, Faculty of Agriculture
and Biotechnology
Data publikacji: 07-07-2021
2018;(594):17-25
SŁOWA KLUCZOWE
STRESZCZENIE
The objective of the study has been to determine the usefulness of six different
Inter Simple Sequence Repeat (ISSR) primers for the genetic stability in the chrysanthemum
plants produced through somatic embryogenesis (SE). The research material consisted of
the lines of two cultivars: ‘Lady Salmon’ (LS1–LS5) and ‘Lady Vitroflora’ (LV1–LV5).
The plants derived from meristems constituted the standard. The primers used in the
PCR reaction have shown a notable polymorphism between the genotypes analyzed. All
the primers (S1–S6) demonstrated a polymorphism in the inter-microsatellite region;
the highest polymorphism in ‘Lady Salmon’ and ‘Lady Vitroflora’ lines with primer S3,
whereas the lowest – in ‘Lady Salmon’ cultivar with primers S5 and S6, and in ‘Lady
Vitroflora’ – with primer S1. UPGMA dendrogram clearly separated all the cultivars and its
lines into two main clusters and two subclusters. ISSR markers can be applied to assess the
genetic stability of SE-derived chrysanthemum.
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| ISSN: | 0084-5477 |
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