The current research investigated 17 species from 5 genera of Cyperaceae. It includes Cyperus, Carex, Kyllinga, Fuirena and Fimbristylis for their foliar epidermal anatomy using light microscopy (LM). The study found that the micromorphological features of the family are important for accurate identification of species. Plant species were collected from different phytogeographical regions of Pakistan and studied for both qualitative and quantitative characteristics. Both the upper and lower epidermis of the leaf were studied, and various micromorphological characters were examined, including the shape of epidermal cells, anticlinal wall pattern, type of stomata, and subsidiary cells. From 5 genera, trichomes were only observed in Kyllinga (Kyllinga brevifolius). All species have paracytic type of stomata. The research aims to develop taxonomic keys based on foliar epidermal characteristics to enable quick and easy identification of species. The main objective is to study both qualitative and quantitative traits for accurate species identification and delimitation of Cyperaceae. The foliar epidermal anatomy of the Cyperaceae family provides valuable information for the systematic identification of different species. The current findings regarding the foliar epidermal features are particularly important for plant taxonomists in accurately identifying species within the Cyperaceae family. The micro-morphological features of foliar epidermal anatomy provide novel characteristics for precise taxonomic identification and offer essential information for further study by plant taxonomists