Acetic acid bacteria K. xylinus are characterized by, i.a., the ability to produce bacterial cellulose, gluconic acid and dihydroxyacetone. The aim of this study was to use non-directional chemical and chemical-physical mutagenisation and to invastigating the impact of this process on the selected phenotypic features of K. xylinus.. Chemical mutagenisation was carried out with the use of methyl methanesulfonate. Chemical-physical mutagenisation has been extended with the application of UV radiation (λ=254 nm). The obtained mutant was characterized by higher of 56% ability to biosynthesize bacterial cellulose (compared to wild type). The study showed that chemical-physical mutagenisation may be a sufficient method for increasing bacterial cellulose synthesis, while it does not ensure an increased yield of gluconic acid and dihydroxyacetone by the tested strain.
ABBREVIATIONS
ADV AGR SCI PROB ISS
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