Biodegradation of cellulose in bottom sediments of Turawa lake
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Publication date: 2007-01-01
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Sławomir Wierzba
Opole University, Chair of Biotechnology and Molecular Biology, ul. Kominka 4, 45-035 Opole
Physicochem. Probl. Miner. Process. 2007;41(1):227-235
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ABSTRACT
In the presented study the attempt at conducting biodegradation of bottom sediments of Turawa Lake was made. In the research cellulolytic bacteria strains were employed and the biodegradation as- sumed 19% reduction of the total cellulose content. At the beginning cellulolitic bacteria, originating from the bottom sediments, were isolated and selected. Next step involved evaluation of the celluloli- tic activity of bacteria strains and selection of the three most vigorous, to be used in the process of bottom sediments biodegradation. The amount of decomposed cellulose was assessed with the use of anthrone method and changes in bacteria enumeration were determined with the index method. The most efficient group, regarding biodegradation of cellulose, was the mixture of the following bacteria species: Cytophaga and Cellulomonas. The reduction obtained was equal to 66%, and the bacteria enumeration increased 300-fold, to finally reach the level of the Most Probable Number equal to 105 NPL per 1g of the bottom sediment.