The objective of the project was a laboratory check of biodegradation of soil samples contaminated by PAH(s), PCB(s) and NEL from the hazardous waste dump in the Pozďátky locality. For the laboratory check, pure bacterial cultures of Rhodococcus sp. and Pseudomonas putida have been used. It is apparent from the laboratory experiments results that after one-month bacterial leaching, applying the bacterium of Rhodococcus sp. there is a 66 % removal of NEL, a 67 % removal of PAH(s) and a 14 % removal of PCB(s). Applying a pure culture of Pseudomonas putida there is a 76 % removal of NEL, a 25 % removal of PAH(s) and a 14 % removal of PCB(s).