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INFLUENCE OF PERMEABILIZATION ON THE CATALYTIC ACTIVITY OF YEAST CELLS YARROWIA LIPOLYTICA KKP 379 IN THE SYNTHESIS OF PLASTICIZER – DIOCTYL ADIPATE
 
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1
WULS-SGGW, Faculty of Food Sciences
 
2
WULS-SGGW, Faculty of Horticulture and Biotechnology
 
3
Institute of Technology of Cambodia, Faculty of Horticulture Biotechnology and Landscape Architecture
 
 
Publication date: 2021-07-06
 
 
2019;(596):23-32
 
KEYWORDS
ABSTRACT
Plasticizers are among the most important additives required for processing of polymer materials. In this paper, biotechnological synthesis of adipic plasticizer – dioctyl adipate was presented with the participation of yeast biomass of Yarrowia lipolytica KKP 379. The reaction was based on transesterification of dimethyl adipate with octan-1-ol. Relative to the whole-cell catalyst were used both physical (treated with glass beads) and chemical (use of isooctane and Triton X-100) methods of permeabilization. The research confirmed the possibility of the synthesis of dioctyl adipate catalyzed by whole cell yeast with the yield 60% (58% ±1,8%). The permeabilization techniques used did not contribute to increasing the reaction efficiency. Among the methods of violating the continuity of the yeast cell wall, the chemical method using Triton X-100 is of interest. Approximately 50% conversion was achieved in this reaction and no decrease in yield was observed during 96 hours of reaction, as opposed to other methods permeabilization.
 
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ISSN:0084-5477
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