In order to find an economical and feasible short process for betafite preconcentrating and to provide a reference for the development of similar low-grade uranium deposits, preconcentration of the betafite ore was investigated based on mineralogical characterization study, float-sink tests, and dense medium cyclone (DMC) separation experiments. The float-sink test results revealed that the gravity separation of the betafite ore was feasible, and the expected particle size range was chosen to be 3~0.3 mm. The effect of important parameters of the DMC experiment such as particle size, grade of the feed, separation density, and inlet pressure on the separation performance of betafite ore was studied. Under the optimal experimental conditions, the expanded experiments were performed and the heavy minerals contained 4557 ppm U and 5200 ppm Nb2O5 with a recovery of 88.86% and 79.73%, respectively, were obtained. Besides, the enrichment ratio (E) values of U and Nb2O5 were 14.24 and 12.78 severally, and the tailings discarding ratio (R) value was 93.76%. The results demonstrate that the preconcentration of low-grade betafite by DMC can remove a large number of tailings and obtain a high-grade uranium concentrate.