To investigate the adhesion of hematite flocs to gas bubbles in floc floatation, this paper develops an observation system for floc-bubble collision and adhesion with two charge-coupled device (CCD) cameras. The sizes of flocs and bubble were 45.36μm and 0.90mm, respectively, and the distance between a floc and the bubble center (sedimentation distance) was set to 0.25cm. Three surfactants, namely, sodium oleate, lauryl amine and sodium dodecyl sulfate (SDS), were selected for our research. Several experiments were conducted to disclose how surfactant concentration and pH affect the surface adhesion between hematite flocs and bubbles. Then, the adhesion mechanism was discussed in details based on the experimental results. The results show that the highest adhesion probability was achieved for the said floc and bubble at the lauryl amine concentration of 8mg/L, the sedimentation distance of 0.25cm and the pH of 9. After touching the bubble, the hermamite floc slid on the bubble surface, forming a stable three-phase interface after 67ms. Then, the radial position of the floc no longer changed, despite the floc motion on the bubble surface. According to the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory and the potential energy of the van der Waals force, there was a repulsive force between the floc and the bubble in the absence of surfactant and an attractive force in the presence of the surfactant of lauryl amine. In addition, a thin solvation shell is conducive to the adhesion between the floc and the bubble.