Oleosomes are lipid carriers and they can carry and protect hydrophobic compounds in hydrophilic environments. They present unique properties and may be applicable in cosmetics and pharmaceutical emulsion. Due to the unique structure, they function as natural emulsifying system which operates over a wide HLB range, allowing you to make sustainable emulsions. Tested formulations with oleosomes showed some interfacial and emulsifying activity. Oleosomes were isolated from safflower seeds (Carthamus tinctorius) and used as an emulsifier for the stabilization of O/W interfaces. Investigated the potential of oleosomes as carriers of active agents’ compounds, including Coenzyme Q10, Omega-3, and Linoleic acid. The study focused on developing natural cosmetic formulations and determining general physicochemical properties as particle size distribution, ζ potential, interfacial tension at the O/W interfaces and evaluating the stability of the resulting formulations.