The study evaluated two micellar biomimetic chromatographic systems for predicting the biological activity of compounds. Drugs with various structures and pharmacological profile were used as test substances. Two chromatographic systems were tested with a C-18 stationary phase and mobile phases: n-propanol and micellar simulated body fluid (SBF) solutions or buffer with a physiological pH of 7.4 at different concentrations of the surfactant SDS. Linear relationships were observed for the retention coefficients k= f(C), log k= f(C) and 1/k= f(C) (C- surfactant concentration). From these relationships, the values of k0, km and KAM were determined and their relationships were examined. The analyzed data showed that the composition of the aqueous mobile phase solution affects interactions within the micellar system. Comparison the obtained values with selected descriptors of biological activity of substances, revealed strong correlations between log KAM values (analogue-micelle association constants) and log KA (HSA) values enabling the prediction of drug binding to proteins based on MLC measurements. In addition, KAM values inform us about potential for drug deposition in micellar systems.