This paper studies the effect of the type and concentration of selected frothers and collectors mix system on the bubble sizes (Sauter mean diameter, SMD) of bubbling flow produced in a micro flotation cell and the determination of bubble size distribution (BSD). The usage of dodecyl amine hydrochloride (DAH) collector on the critical coalescence concentration of commercial frothers PPG200, PPG400, and PPG600 was investigated in detail. The results of these studies showed that the usage of DAH decreased the CCC of these frothers. Each frother + collector mixing system exhibited its unique ability in preventing coalescence of the bubbles in the order of PPG200 < PPG400 < PPG600. The factorial experiments established that the type of the frother, collector, and their concentration had a major effect on the size of the bubbles. The BSD in the presence of PPG600 + DAH mix system resulted in a little bit wider BSD which indicated the effect of frother type in mixed systems.