The influence of flotation reagents on sulfur-oxidizing bacteria Acidithiobacillus thiooxidans
 
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1
Department of Metallurgy, Silesian University of Technology
2
Environmental Biotechnology Department, Silesian University of Technology
3
University of Silesia
CORRESPONDING AUTHOR
Malgorzata Pacholewska   

Department of Metallurgy, Silesian University of Technology, Krasinskiego 8, 40-019 Katowice, Poland
Publish date: 2008-01-01
 
Physicochem. Probl. Miner. Process. 2008;42(1):37–46
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ABSTRACT
It has been demonstrated that various flotation reagents influence, in a different manner, the meta- bolic activity of the sulfur-oxidizing bacteria Acidithiobacillus thiooxidans (strain C1 being isolated from the Fe-Zn tailings water) growing in the Waksman/Joffe (W/J) liquid culture medium that con- tains thiosulfate as a sole energy source for bacteria growth. The ethyl- and amyl xanthates as well as the frothing reagent stimulated, to a limited extent, the tested C1-bacteria metabolic activity. The very well documented bacterially-influenced acidification of the W/J solution supplemented with the ethyl- or the amyl xanthates suggests the possibility of these substances effective acid-degradation in the post-industrial environments rich in various flotation reagents, mainly xanthates. Both the activator containing the carbamate ethyl-derivative and the modifier composed of Cu(II)-ions caused a complete inactivation of the A. thiooxidans C1-metabolism. It is suggested that some unexpected chemi- cal reactions may proceed in the tested systems, as a result of interactions between the culture medium components, flotation reagents, their decomposition products, and also the products of bacterial metabolism.
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