Settling process is used to separate solid particles from water suspension. Many factors, like the presence of chemical compounds, affect this process. In this paper, the effect of surfactants addition on the settling velocity of magnesite solid waste was investigated. The studies were conducted in the presence of a natural surfactant (rhamnolipid from Jeneil Biosurfactant Co), cell culture filtrate of Pseudomonas aeruginosa, and a chemical surfactant (sodium dodecyl sulphate (SDS)). The addition of these compounds caused destabilization of the investigated suspension. A particle size increase and rapid sedimentation were observed. It was noted that the addition of biosurfactant caused a faster settling velocity in comparison to the SDS addition.