HYDROXYAPATITE AS A SUPPORT IN PROTEASE IMMOBILIZATION PROCESS
 
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Poznan University of Technology
 
 
Publication date: 2015-03-26
 
 
Corresponding author
Teofil Jesionowski   

Poznan University of Technology, Berdychowo 4, 60-695 Poznan, Poland
 
 
Physicochem. Probl. Miner. Process. 2021;(unknown)
 
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ABSTRACT
Hydroxyapatite (HA) is used as a matrix for immobilization of protease from Aspergillus oryzae by the process of adsorption. The matrix obtained has the surface area of 26 m2/g and particles in the shape of flakes of diameters no greater than 650 nm. The efficiency of the method proposed was confirmed by Fourier transform infrared spectroscopy (FTIR), elemental analysis (AE) and by analysis of parameters of the pore structure of the matrix and the products after immobilization. On the basis of the Bradford method it was found that the greatest amount of the enzyme (132 mg/g) was immobilized from the solution of initial enzyme concentration of 7 mg/mL after 24 h of the process.
eISSN:2084-4735
ISSN:1643-1049
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