Field experiment was conducted in 2013 in Duninowo (54o539’ N, 16o830’ E). The experimental factors were: I. factor - 6 variants of fertilization, and II. - two level of EM preparations. The aim of this study was to evaluate the impact of ash from biomass by comparing its effect with the calcium fertilizer and compost BIOTOPE in conjunction with the preparation of microbiological Effective Microorganisms (EM). The impact of ash from biomass introduced into the soil on yield and yield structure and physiological parameters of spring wheat was analyzed No significant impact of the various variants of fertilizer application on the yielding of spring wheat cv.Bombona was confirmed. As a result of the form of compost fertilizer BIOTOPE, an increase in the content of chlorophyll in leaves of wheat cv Bombona (SPAD) and the size of canopy assimilation area per unit area of the field (LAI). The application of EM did not affect the physiological parameters (yield, the number of ears per area unit, SPAD, LAI) characterizing the spring wheat cv. Bombona.