In this paper, continuous flow analyzer has been examined for the usefulness for the determination of anionic surfactants and nonionics in real water and sewage samples. The study used a segmented flow analyzer (SFA) SAN++ (Skalar, Netherlands) with photometric detection. Environmental water and sewage samples of different origin and standard solutions were under test. By appropriate selection of analytical conditions in continuous flow system, it has been possible to obtain satisfactory values of the significant characteristics of applied methods for the determination of both surfactants. On the basis of the standard addition it was confirmed that proposed continuous flow system is useful for the determination of the surfactants also in materials with complicated matrix. Established methods show low limit of detection, good precision and good correctness. The described full automatic method takes effect in short-time analysis, small sample volume required for testing and waste restriction. Proposed flow injection system comply with requirements and may be successfully applied in monitoring studies as well as in the routine laboratory analysis. Rapid determination of water and waste water quality by the SFA for the content of surfactants allows an adequate response in case of exceeding the permissible concentrations, even according to the most restricted requirements.