Manufacturing companies are now placing great emphasis on competitiveness and looking for ways to explore their resources more efficiently. This paper presents optimum efficiency improvement of the automotive transmission assembly production line by using line balancing. To optimize has been selected 3 assembly stations where is waste and where requirements are not met for achieving the production capacity. Several measures have been proposed on the assembly lines concerned to reduce operations by using eliminating unnecessary activities of the assembly processes, reducing the cycle time, and balancing manpower workload using line balancing through Yamazumi chart and Takt time. The results of the proposed measures were compared with the current situation in terms of increasing the efficiency of the production line.