RESEARCH PAPER
Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis
1
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Corresponding author
Oľga Danišová
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Ann Agric Environ Med. 2016;23(2):254-258
KEYWORDS
ABSTRACT
Introduction:
In this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the PCR methods: nested PCR for amplification of SSU region; nested PCR for amplification of GP60 region; and with restriction analysis of DNA (PCR-RFLP). The sequencing identified the following species: Cryptosporidium muris (2), Cryptosporidium andersoni (1), Cryptosporidium bovis (4), Cryptosporidium suis (2), Cryptosporidium scrofarum (10), mixed infection caused by C. scrofarum and C. muris (1),and Cryptosporidium parvum (10) genotype A subtype IIaA17G2R1.
Results and conclusions:
The findings suggest that livestock can be an important source of zoonotic species or genotypes of Cryptosporidium, which may adversely affect the public health of human populations. This is the first time in our country that the Cryptosporidium species has been identified in livestock in Slovakia. The identification and genotyping of this pathogen in Slovakia, completes the epidemiological situation in Europe for Cryptosporidum species.
In this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the PCR methods: nested PCR for amplification of SSU region; nested PCR for amplification of GP60 region; and with restriction analysis of DNA (PCR-RFLP). The sequencing identified the following species: Cryptosporidium muris (2), Cryptosporidium andersoni (1), Cryptosporidium bovis (4), Cryptosporidium suis (2), Cryptosporidium scrofarum (10), mixed infection caused by C. scrofarum and C. muris (1),and Cryptosporidium parvum (10) genotype A subtype IIaA17G2R1.
Results and conclusions:
The findings suggest that livestock can be an important source of zoonotic species or genotypes of Cryptosporidium, which may adversely affect the public health of human populations. This is the first time in our country that the Cryptosporidium species has been identified in livestock in Slovakia. The identification and genotyping of this pathogen in Slovakia, completes the epidemiological situation in Europe for Cryptosporidum species.
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